Functional characterization of maize heat shock transcription factor gene ZmHsf01 in thermotolerance

PeerJ

article

Huaning Zhang¹  Guoliang Li¹  Dong Hu¹  Yuanyuan Zhang²  Yujie Zhang³  Hongbo Shao⁴  Lina Zhao²  Ruiping Yang⁵  Xiulin Guo¹ 

[1] Plant Genetic Engineering Center of Hebei Province/Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences;College of Life Science, Hebei Normal University;College of Agriculture and Forestry Science and Technology, Hebei North University;Salt-soil Agricultural Center, Key Laboratory of Agricultural Environment in the Lower Reaches of Yangtze River Plain, Institute of Agriculture Resources and Environment, Jiangsu Academy of Agricultural Sciences;Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Yancheng Teachers University;College of Environment and Safety Engineering, Qingdao University of Science & Technology

关键词: Thermotolerance;    Heat shock transcription factors;    Maize;    ABA;    Subcellular localization;    H2O2;    Adversity stresses;    Signal transduction pathway;    Functional analysis;    ZmHsf01;   

DOI  :  10.7717/peerj.8926

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundHeat waves can critically influence maize crop yields. Plant heat shock transcription factors (HSFs) play a key regulating role in the heat shock (HS) signal transduction pathway.MethodIn this study, a homologous cloning method was used to clone HSF gene ZmHsf01 (accession number: MK888854) from young maize leaves. The transcript levels of ZmHsf01 were detected using qRT-PCR in different tissues and treated by HS, abscisic acid (ABA), hydrogen peroxide (H2O2), respectively, and the functions of gene ZmHsf01 were studied in transgenic yeast and Arabidopsis.ResultZmHsf01 had a coding sequence (CDS) of 1176 bp and encoded a protein consisting of 391 amino acids. The homologous analysis results showed that ZmHsf01 and SbHsfA2d had the highest protein sequence identities. Subcellular localization experiments confirmed that ZmHsf01 was localized in the nucleus. ZmHsf01 was expressed in many maize tissues. It was up-regulated by HS, and up-regulated in roots and down-regulated in leaves under ABA and H2O2treatments. ZmHsf01-overexpressing yeast cells showed increased thermotolerance. In Arabidopsis seedlings, ZmHsf01 compensated for the thermotolerance defects of mutant athsfa2, and ZmHsf01-overexpressing lines showed enhanced basal and acquired thermotolerance. When compared to wild type (WT) seedlings, ZmHsf01-overexpressing lines showed higher chlorophyll content and survival rates after HS. Heat shock protein (HSP) gene expression levels were more up-regulated in ZmHsf01-overexpressing Arabidopsis seedlings than WT seedlings. These results suggest that ZmHsf01 plays a vital role in response to HS in plant.

【 授权许可】

CC BY