Mitochondrial small heat‐shock protein enhances thermotolerance in tobacco plants

FEBS Letters

Egawa, Yoshinobu¹  Sanmiya, Kazutsuka¹  Shono, Mariko¹  Suzuki, Katsumi¹ 

[1] Okinawa Subtropical Station, Japan International Research Center for Agricultural Sciences, Ishigaki, Okinawa 9070002, Japan

关键词: Mitochondrial small heat-shock protein;    Thermotolerance;    Tobacco;    Transformation;    GST;    glutathione S-transferase;    MS;    Murashige and Skoog;    MT-sHSP;    mitochondrial small heat-shock protein;   

DOI  :  10.1016/S0014-5793(03)01494-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To clarify the role of mitochondrial small heat-shock protein (MT-sHSP) in the heat-shock response, we introduced the tomato (Lycopersicon esculentum) MT-sHSP gene under the control of the 35S promoter into tobacco (Nicotiana tabacum), and examined the thermotolerance of the transformed plants. Irrespective of the orientation, sense or antisense, of the gene, the transgenic plants exhibited a normal morphology and growth rate in the vegetative growth stage. When 4-week-old seedlings were exposed to sudden heat stress, the sense plants which overexpress the MT-sHSP gene exhibited thermotolerance, whereas the antisense plants in which the expression of the gene is suppressed exhibited susceptibility.

【 授权许可】

Unknown