Genome-wide characterization of the Zn(II) 2 Cys 6 zinc cluster-encoding gene family in Pleurotus ostreatus and expression analyses of this family during developmental stages and under heat stress

PeerJ

article

Zhihao Hou¹  Qiang Chen¹  Mengran Zhao¹  Chenyang Huang¹  Xiangli Wu¹ 

[1] Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences;Key Laboratory of Microbial Resources, Ministry of Agriculture and Rural Affairs

关键词: Zinc cluster protein;    Pleurotus ostreatus;    Fruiting body development;    Heat stress;   

DOI  :  10.7717/peerj.9336

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Pleurotus ostreatus is one of the most widely cultivated mushrooms in China. The regulatory mechanisms of fruiting body formation and the response to heat stress in P. ostreatus are main research focuses. The Zn(II)2Cys6 family is one of the largest families of transcriptional factors and plays important roles in multiple biological processes in fungi. In this study, we identified 66 zinc cluster proteins in P. ostreatus (PoZCPs) through a genome-wide search. The PoZCPs were classified into 15 types according to their zinc cluster domain. Physical and chemical property analyses showed a huge diversity among the PoZCPs. Phylogenetic analysis of PoZCPs classified these proteins into six groups and conserved motif combinations and similar gene structures were observed in each group. The expression profiles of these PoZCP genes during different developmental stages and under heat stress were further investigated by RNA-sequencing (RNA-seq), revealing diverse expression patterns. A total of 13 PoZCPs that may participate in development or the heat stress response were selected for validation of their expression levels through real-time quantitative PCR (RT-qPCR) analysis, and some developmental stage-specific and heat stress-responsive candidates were identified. The findings contribute to our understanding of the roles and regulatory mechanisms of ZCPs in P. ostreatus.

【 授权许可】

CC BY