Highly diverse root endophyte bacterial community is driven by growth substrate and is plant genotype-independent in common bean ( Phaseolus vulgaris L.)

PeerJ

article

Aarón Barraza¹  Juan Carlos Vizuet-de-Rueda²  Raúl Alvarez-Venegas² 

[1] Programa de Agricultura en Zonas Áridas;Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional

关键词: Bacterial community structure;    Growth substrates;    Next-generation sequencing;    Root inner bacteria;    Root genotype;   

DOI  :  10.7717/peerj.9423

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The common bean (Phaseolus vulgaris L.) is the most important grain legume in the human diet with an essential role in sustainable agriculture mostly based on the symbiotic relationship established between this legume and rhizobia, a group of bacteria capable of fixing atmospheric nitrogen in the roots nodules. Moreover, root-associated bacteria play an important role in crop growth, yield, and quality of crop products. This is particularly true for legume crops forming symbiotic relationships with rhizobia, for fixation of atmospheric N2. The main objective of this work is to assess the substrate and genotype effect in the common bean (Phaseolus vulgaris L.) root bacterial community structure. To achieve this goal, we applied next-generation sequencing coupled with bacterial diversity analysis. The analysis of the bacterial community structures between common bean roots showed marked differences between substrate types regardless of the genotype. Also, we were able to find several phyla conforming to the bacterial community structure of the common bean roots, mainly composed by Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, and Firmicutes. Therefore, we determined that the substrate type was the main factor that influenced the bacterial community structure of the common bean roots, regardless of the genotype, following a substrate-dependent pattern. These guide us to develop efficient and sustainable strategies for crop field management based on the soil characteristics and the bacterial community that it harbors.

【 授权许可】

CC BY