Identification of novel mutations in RNA-dependent RNA polymerases of SARS-CoV-2 and their implications on its protein structure

PeerJ

article

Gyanendra Bahadur Chand¹  Atanu Banerjee²  Gajendra Kumar Azad¹ 

[1] Department of Zoology, Patna University;Department of Zoology, Samastipur College

关键词: COVID-19;    SARS-CoV-2;    RNA-dependent RNA polymerases (RdRp);    Nsp12;    Mutation;    Indian geographical area;   

DOI  :  10.7717/peerj.9492

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The rapid development of the SARS-CoV-2 mediated COVID-19 pandemic has been the cause of significant health concern, highlighting the immediate need for effective antivirals. SARS-CoV-2 is an RNA virus that has an inherently high mutation rate. These mutations drive viral evolution and genome variability, thereby facilitating viruses to have rapid antigenic shifting to evade host immunity and to develop drug resistance. Viral RNA-dependent RNA polymerases (RdRp) perform viral genome duplication and RNA synthesis. Therefore, we compared the available RdRp sequences of SARS-CoV-2 from Indian isolates and the ‘Wuhan wet sea food market virus’ sequence to identify, if any, variation between them. Our data revealed the occurrence of seven mutations in Indian isolates of SARS-CoV-2. The secondary structure prediction analysis of these seven mutations shows that three of them cause alteration in the structure of RdRp. Furthermore, we did protein modelling studies to show that these mutations can potentially alter the stability of the RdRp protein. Therefore, we propose that RdRp mutations in Indian SARS-CoV-2 isolates might have functional consequences that can interfere with RdRp targeting pharmacological agents.

【 授权许可】

CC BY