Increasing methane (CH 4 ) emissions and altering rhizosphere microbial diversity in paddy soil by combining Chinese milk vetch and rice straw

PeerJ

article

Qiaoying Ma¹  Jiwei Li¹  Muhammad Aamer¹  Guoqin Huang¹ 

[1] Research Center on Ecological Sciences, Jiangxi Agricultural University

关键词: Chinese Milk Vetch;    Methane(CH4) emission;    16S rDNA;    ITS1;    Microbial community diversity;   

DOI  :  10.7717/peerj.9653

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundChinese milk vetch (Astragalus sinicus L.) can improve paddy soil fertility and ecology through nitrogen fixation, but it can also increase greenhouse gas emissions. Our primary objective was to investigate how Chinese milk vetch, rice straw, and nitrogen fertilization affect the methane and microbial components of the rice rhizosphere.MethodsWe examined the rhizosphere’s methane emissions and microbial abundance and diversity after incorporating Chinese milk vetch and rice straw into paddy soil. We used high-throughput sequencing of the 16s rRNA and ITS1 genes to study changes in the bacterial and fungal communities, respectively. Over the course of our experiment, we applied seven different treatments to the paddy soil: conventional fertilization (the control treatment) for winter fallow crops, three levels of nitrogen in Chinese milk vetch, and three levels of nitrogen in Chinese milk vetch combined with rice straw.ResultsRice yield and methane emissions increased during cultivation when the soil was treated with Chinese milk vetch with and without added straw. The nitrogen application also affected the methane fluxes. Alpha diversity measurements showed that Chinese milk vetch increased the diversity of the soil fungal community but did not significantly affect the bacterial community. Chinese milk vetch affected the rhizosphere microorganism communities by increasing the number of Methanomicrobia.

【 授权许可】

CC BY