Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Journal of Veterinary Medical Science

Toshiyuki SHIBAHARA⁴  Koichi FUKUDA¹  Tadashi ITAGAKI³  Kunio TERASAKI²  Masayuki KIKAWA³ 

[1] Center for Laboratory Animal Science, National Defense Medical College;St. Mary's Junior College;Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University;Division of Laboratory Animal Science, Research Center for Bioscience and Technology, Tottori University

关键词: Fasciola sp.;    haplotype;    ITS1;    Korea;    NDI;   

DOI  :  10.1292/jvms.67.1115

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(44)Nucleotide sequences of ribosomal internal transcribed spacer (ITS1) and mitochondrial NADH dehydrogenase I (NDI) gene were analyzed to genetically characterize aspermic Fasciola forms in Korea. From the difference in ITS1 sequences, Korean flukes were divided into 3 haplotypes represented by Kor1, Kor2 and Kor1/2, which had nucleotides identical to F. hepatica, F. gigantica and those overlapped between the two species, respectively. NDI sequences also showed that Korean flukes could be classified into 3 distinct haplotypes (Kor1: F. hepatica-type, Kor2a and Kor2b: F. gigantica-type). The sequences of Kor1 and Kor2a were 100% identical to those of the haplotypes Fsp1and Fsp2, respectively, which are major Fasciola forms in Japan. These findings strongly suggest that aspermic Fasciola forms in Korea and Japan originated from same ancestors and have recently spread throughout both countries.

【 授权许可】

Unknown