Sequence differences in the internal transcribed spacer 1 and 5.8S ribosomalRNA among three Moniezia species isolated from ruminants inJapan

Journal of Veterinary Medical Science

Maiko OHTORI¹  Mikiko AOKI¹  Tadashi ITAGAKI¹ 

[1] Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda 3�?18�?8, Morioka 020�?8550, Japan

关键词: 5.8S rRNA;    ITS1;    Moniezia benedeni;    Moniezia expansa;    Moniezia monardi;    phylogeny;   

DOI  :  10.1292/jvms.14-0309

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(1)This study was designed to clarify the differences in the internal transcribedspacer (ITS) 1 and 5.8S nucleotide sequences of Moniezia expansa,M. benedeni and M. monardi isolated from ruminants inJapan and to determine their phylogenetic relationships. A 98% similarity in the 5.8Ssequences was observed among the 3 Moniezia species, whereas manynucleotide indels and substitutions were observed in the ITS1 sequences among the threeMoniezia species. These results suggest that the ITS1 region couldserve as a potential marker for discriminating the 3 Moniezia species. Inthe phylogenetic tree based on the ITS1 sequences, M. monardi andM. benedeni showed genetically closer relationship to each other thanto M. expansa.

【 授权许可】

Unknown