Extracellular vesicles in patients in the acute phase of psychosis and after clinical improvement: an explorative study

PeerJ

article

Mette Elise Tunset¹  Hanne Haslene-Hox³  Tim Van Den Bossche⁴  Arne Einar Vaaler¹  Einar Sulheim³  Daniel Kondziella⁷ 

[1] Department of Østmarka- Division of Mental Healthcare, St. Olavs University Hospital;Department of Mental Health- Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology;Department of Biotechnology and Nanomedicine;VIB - UGent Center for Medical Biotechnology;Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University;Department of Physics, Norwegian University of Science and Technology;Department of Neurology, Rigshospitalet, Copenhagen University Hospital;Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen

关键词: Psychosis;    Proteomics;    Extracellular vesicles;    Gene ontology;    Blood brain barrier;    Glymphatics;    Substance abuse;    Immune system;    Glutamatergic synapses;    Brain enriched proteins;   

DOI  :  10.7717/peerj.9714

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Extracellular vesicles (EVs) are cell-derived structures that transport proteins, lipids and nucleic acids between cells, thereby affecting the phenotype of the recipient cell. As the content of EVs reflects the status of the originating cell, EVs can have potential as biomarkers. Identifying EVs, including their cells of origin and their cargo, may provide insights in the pathophysiology of psychosis. Here, we present an in-depth analysis and proteomics of EVs from peripheral blood in patients (n = 25) during and after the acute phase of psychosis. Concentration and protein content of EVs in psychotic patients were twofold higher than in 25 age- and sex-matched healthy controls (p < 0.001 for both concentration and protein content), and the diameter of EVs was larger in patients (p = 0.02). Properties of EVs did not differ significantly in blood sampled during and after the acute psychotic episode. Proteomic analyses on isolated EVs from individual patients revealed 1,853 proteins, whereof 45 were brain-elevated proteins. Of these, five proteins involved in regulation of plasticity of glutamatergic synapses were significantly different in psychotic patients compared to controls; neurogranin (NRGN), neuron-specific calcium-binding protein hippocalcin (HPCA), kalirin (KALRN), beta-adducin (ADD2) and ankyrin-2 (ANK2). To summarize, our results show that peripheral EVs in psychotic patients are different from those in healthy controls and point at alterations on the glutamatergic system. We suggest that EVs allow investigation of blood-borne brain-originating biological material and that their role as biomarkers in patients with psychotic disorders is worthy of further exploration.

【 授权许可】

CC BY