The genetic diversity and population structure of two endemic Amazonian quillwort ( Isoetes L.) species

PeerJ

article

Mirella Pupo Santos¹  João V.S. Rabelo Araujo¹  Arthur V. Sant’anna Lopes¹  Julio Cesar Fiorio Vettorazzi²  Marcela Santana Bastos Boechat²  Fernanda AbreuSantana Arêdes²  Naiara Viana Campos¹  Emiliano Nicolas Calderon¹  Fernando M. Gomes Santos³  Tais Nogueira Fernandes³  Rodrigo Nunes da Fonseca¹  Messias Gonzaga Pereira²  Guilherme Oliveira⁴  Daniel Basilio Zandonadi¹  RodrigoLemes Martins¹  Francisco de Assis Esteves¹ 

[1] Instituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro;Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense;Environmental Studies Office;Instituto Tecnológico Vale

关键词: Isoetes cangae;    Isoetes serracarajensis;    ISSR;    Lycophyte;    Gene flow;    Mating system;   

DOI  :  10.7717/peerj.10274

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundTwo endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies.MethodsThe genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated.ResultsThe sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species.

【 授权许可】

CC BY