The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species

PeerJ

卷:8

Fernando M. Gomes Santos¹  Tais Nogueira Fernandes¹  Guilherme Oliveira²  Francisco de Assis Esteves³  Emiliano Nicolas Calderon³  Naiara Viana Campos³  RodrigoLemes Martins³  João V.S. Rabelo Araujo³  Daniel Basilio Zandonadi³  Rodrigo Nunes da Fonseca³  Mirella Pupo Santos³  Arthur V. Sant’anna Lopes³  Marcela Santana Bastos Boechat⁴  Fernanda AbreuSantana Arêdes⁴  Messias Gonzaga Pereira⁴  Julio Cesar Fiorio Vettorazzi⁴ 

[1] Environmental Studies Office, Vale, Belo Horizonte, Minas Gerais, Brazil;

[2] Instituto Tecnológico Vale, Belém, Pará, Brazil;

[3] Instituto de Sustentabilidade e Biodiversidade, Universidade Federal do Rio de Janeiro, Macaé, Rio de Janeiro, Brazil;

[4] Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil;

关键词: Isoetes cangae;    Isoetes serracarajensis;    ISSR;    Lycophyte;    Gene flow;    Mating system;   

DOI  :  10.7717/peerj.10274

来源: DOAJ

【 摘 要 】

Background Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies. Methods The genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated. Results The sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species.

【 授权许可】

Unknown