Effects of platelet-rich plasma on mesenchymal stem cells isolated from rat uterus

PeerJ

article

Polina Vishnyakova¹  Daria Artemova¹  Andrey Elchaninov¹  Zulfiia Efendieva⁵  Inna Apolikhina¹  Gennady Sukhikh¹  Timur Fatkhudinov² 

[1] National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation;Peoples’ Friendship University of Russia ,(RUDN University);Scientific Research Institute of Human Morphology;Pirogov Russian National Research Medical University;I. M. Sechenov First Moscow State Medical University of Ministry of Health of Russia ,(Sechenov University)

关键词: Mesenchymal stem cells;    Platelet-rich plasma;    Rat uterine;    Plasma;    Endometrium;   

DOI  :  10.7717/peerj.10415

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Platelet-rich plasma (PRP), which represents a valuable source of growth factors, is increasingly being applied in regenerative medicine. Recent findings suggest the feasibility of using PRP in the treatment of infertility secondary to refractory thin endometrium. Mesenchymal stem/stromal cells (MSCs) of the endometrium are an essential cellular component responsible for extracellular matrix remodeling, angiogenesis, cell-to-cell communication, and postmenstrual tissue repair. Using a rat model, we examine the effects of autologous PRP on MSCs isolated from the uterus and compare them with the effects of autologous ordinary plasma (OP) and complete growth medium. Methods MSCs were isolated from uterine tissues via enzymatic disaggregation. Flow cytometry immunophenotyping of the primary cell cultures was complemented by immunocytochemistry for Ki-67 and vimentin. The ability of MSCs to differentiate in osteo-, chondro-, and adipogenic directions was assessed using differentiation-inducing media. The levels of autophagy and apoptosis markers, as well as the levels of matrix metalloproteinase 9 (MMP9) and estrogen receptor α, were assessed by western blotting. Results After 24 h incubation, the proliferation index of the PRP-treated MSC cultures was significantly higher than that of the MSC cultures treated with complete growth medium. PRP treatment elevated production of LC3B protein, an autophagy marker, while OP treatment upregulated the expression of stress-induced protein p53 and extracellular enzyme MMP9. The results indicate practical relevance and validity for PRP use in the treatment of infertility.

【 授权许可】

CC BY