Combination of circulating miR-145-5p/miR-191-5p as biomarker for breast cancer detection

PeerJ

article

Yeldar Ashirbekov¹  Arman Abaildayev¹  Nazgul Omarbayeva²  Dauren Botbayev¹  Ayaz Belkozhayev¹  Anel Askandirova²  Alena Neupokoyeva³  Gulzhakhan Utegenova⁴  Kamalidin Sharipov¹  Nagima Aitkhozhina¹ 

[1] M. Aitkhozhin Institute of Molecular Biology and Biochemistry;Kazakh Research Institute of Oncology and Radiology;Almaty Branch of National Center for Biotechnology;South Kazakhstan State Pedagogical University

关键词: Breast cancer;    Circulating miRNA;    Biomarker;    Plasma;    miR-145;    miR-191;    miR-21;    Diagnosis;    Kazakh population;   

DOI  :  10.7717/peerj.10494

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundBreast cancer (BC) is the most common cancer among women worldwide. At present, there is a need to search for new, accurate, reliable, minimally invasive and cheap biomarkers in addition to existing methods for the diagnosis and prognosis of BC. The main goal of this study was to test the diagnostic value of six circulating miRNAs in Kazakh women.Materials and methodsTaqMan-based miRNA profiling was conducted using plasma specimens from 35 BC women patients and 33 healthy women samples (control group).ResultsThe level of all seven miRNAs (including endogenous control) normalized by synthetic cel-miR-39 were significantly elevated in the group of BC patients. Normalization using miR-222-3p as endogenous control reduced differences in level of miRNAs between groups; as a result, only three miRNAs were significantly upregulated in the group of BC patients—miR-145-5p (P = 6.5e−12), miR-191-5p (P = 3.7e−10) and miR-21-5p (P = 0.0034). Moreover, ROC analysis showed that the use of miR-145-5p and miR-191-5p, both individually (AUC = 0.931 and 0.904, respectively) or in combination (AUC = 0.984), allows to accurately differentiate BC patients from healthy individuals.ConclusionsTwo plasma miRNAs—miR-145-5p and miR-191-5p—are potential biomarkers for diagnosis of BC in the Kazakh population. The findings need to be further substantiated using a more representative sample.

【 授权许可】

CC BY