Expression of vitamin D 1α-hydroxylase in human gingival fibroblasts in vivo

PeerJ

article

Kaining Liu¹  Bing Han²  Jianxia Hou¹  Jianyun Zhang²  Jing Su⁷  Huanxin Meng¹ 

[1] Department of Periodontology, Peking University School and Hospital of Stomatology;National Clinical Research Center for Oral Diseases;National Engineering Laboratory for Digital and Material Technology of Stomatology;Beijing Key Laboratory of Digital Stomatology;Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology;Department of Oral Pathology, Peking University School and Hospital of Stomatology;Department of Pathology, Peking University Health Science Center

关键词: 25-hydroxyvitamin D3 1-alpha-hydroxylase;    Gene expression;    Gingiva;    Periodontitis;   

DOI  :  10.7717/peerj.10279

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundVitamin D 1α-hydroxylase CYP27B1 is the key factor in the vitamin D pathway. Previously, we analyzed the expression of CYP27B1 in human gingival fibroblasts in vitro. In the present study, we analyzed the gingival expression of CYP27B1 in vivo.MethodsForty-two patients with periodontitis Stage IV Grade C and 33 controls were recruited. All patients with periodontitis had unsalvageable teeth and part of the wall of the periodontal pocket was resected and obtained after tooth extraction. All controls needed crown-lengthening surgery, and samples of gingiva resected during surgery were also harvested. All the individuals’ gingivae were used for immunohistochemistry and immunofluorescence. In addition, gingivae from seventeen subjects of the diseased group and twelve subjects of the control group were analyzed by real-time PCR.ResultsExpression of CYP27B1 was detected both in gingival epithelia and in gingival connective tissues, and the expression in connective tissues colocalized with vimentin, indicating that CYP27B1 protein is expressed in gingival fibroblasts. The expression of CYP27B1 mRNA in gingival connective tissues and the CYP27B1 staining scores in gingival fibroblasts in the diseased group were significantly higher than those in the control group.ConclusionsExpression of CYP27B1 in human gingival tissues was detected, not only in the fibroblasts of gingival connective tissues, but also in the gingival epithelial cells, and might be positively correlated with periodontal inflammation.

【 授权许可】

CC BY