Periodontal CD14 mRNA expression is downregulated in patients with chronic periodontitis and type 2 diabetes

BMC Oral Health

Yan Huang¹  Joe W. Krayer³  Renata S. Leite⁵  Junfei Jin⁴  Xiaoming Zhang²  Dustin C. Hedgpeth³ 

[1] Ralph H. Johnson VA Medical Center, 114 Doughty Street, Charleston 29401, SC, USA;Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, College of Medicine, Medical University of South Carolina, 114 Doughty Street, Charleston 29425, SC, USA;Department of Stomatology, James B. Edwards College of Dental Medicine, Medical University of South Carolina, Charleston 29425, SC, USA;Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi, People’s Republic of China;Center for Oral Health Research, James B. Edwards College of Dental Medicine, Medical University of South Carolina, Charleston 29425, SC, USA

关键词: Gene expression;    Diabetes;    TLR4;    CD14;    Periodontitis;   

Others  :  1233465
DOI  :  10.1186/s12903-015-0118-3

 received in 2015-05-17, accepted in 2015-10-15,  发布年份 2015

【 摘 要 】

Background

Patients with type 2 diabetes mellitus (T2DM) have increased severity of periodontitis. Toll-like receptor (TLR)4, its co-receptors CD14 and MD-2, and adaptor MyD88 play pivotal roles in lipopolysaccharide (LPS)-triggered tissue inflammation and periodontitis. This study investigated the effects of T2DM and periodontitis on TLR4, CD14, MD-2 and MyD88 mRNA expression in surgically removed periodontal tissues.

Methods

Periodontal tissue specimens were collected from 14 patients without periodontitis and T2DM (Group 1), 15 patients with periodontitis alone (Group 2), and 7 patients with both periodontitis and T2DM (Group 3). The mRNA of TLR4, CD14, MD-2 and MyD88 was quantified using real-time PCR and compared between the groups.

Results

Statistical analysis showed that periodontal expression of CD14 mRNA was significantly reduced across Groups 1, 2 and 3 (p = 0.02) whereas the mRNA expression of TLR4, MD-2 and MyD88 was not significantly different among the groups. Furthermore, when patients in Groups 1 and 2 were combined (n = 22), the CD14 mRNA expression was significantly lower than that in patients of Group 1 (p = 0.04).

Conclusions

CD14 mRNA expression was downregulated across patients with neither periodontitis nor T2DM, patients with periodontitis alone and patients with both diseases, suggesting that CD14 mRNA expression is associated with a favorable host response or subjected to a negative feedback regulation.

【 授权许可】

   
2015 Hedgpeth et al.

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