The association of polymorphisms of TLR4 and CD14 genes with susceptibility to sepsis in a Chinese population

BMC Medical Genetics

Liao Pinhu²  Suren R Sooranna⁴  Xiaowen Qiu²  Donghai Hu²  Jun Li²  Gang Qin³  Bin Xiong¹  Yueqiu Qin²  Yi Zeng²  Yesheng Wei²  Haiyan Wang² 

[1] Intensive Care Unit, the People’s Hospital of Guangxi Zhuang Autonomous Region, 6 Taoyuan Road, Nanning 530021, Guangxi, PR China;Intensive Care Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, No.18 Zhongshan Road II, Baise 533099, Guangxi, PR China;Intensive Care Unit, Minzu Hospital of Guangxi Zhuang Autonomous Region, 323 Mingxiu East Road, Nanning 530021, Guangxi, PR China;Department of Surgery and Cancer, Imperial College London, Chelsea and Westminster Hospital, London SW10 9NH, UK

关键词: CD14;    TLR4;    Single nucleotide polymorphism;    Sepsis;   

Others  :  1090217
DOI  :  10.1186/s12881-014-0123-4

 received in 2014-07-02, accepted in 2014-10-23,  发布年份 2014

【 摘 要 】

Background

Sepsis is now the leading cause of death in the non-cardiovascular intensive care unit (ICU). Recent research suggests that sepsis is likely to be due to an interaction between genetic and environmental factors. Genetic mutations of toll-like receptor 4 (TLR4) and cluster of differentiation 14 (CD14) genes are involved in the immune and (or) inflammatory response. These may contribute to the susceptibility to sepsis in patients. This study was designed to evaluate whether the TLR4 and cluster CD14 gene polymorphisms are associated with susceptibility to sepsis.

Methods

The single nucleotide polymorphisms (SNPs) of TLR4 (rs10759932, rs11536889, rs7873784, rs12377632, rs1927907, rs1153879) and CD14 (rs2569190 and rs2563298) in patients with sepsis and control subjects in the Guangxi Province were analyzed by using the polymerase chain reaction-single base extension (PCR-SBE) and DNA sequencing methods.

Results

The rs11536889 polymorphism in TLR4 and rs2563298 polymorphism in CD14 were significantly associated with the risk of sepsis when compared to the control group. The frequencies of rs11536889 and rs2563298 polymorphisms in the group with sepsis were higher than that in the control group (OR = 1.430, 95% CI, 1.032-1.981, P<0.05; OR = 2.454, 95% CI, 1.458-4.130, P<0.05, respectively). Followed up haplotype analysis suggested that there were two haplotypes in which increased risk factors for sepsis were indicated.

Conclusions

The rs11536889 polymorphism in TLR4 and rs2563298 polymorphism in CD14, and two haplotypes were associated with increased susceptibility to sepsis.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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