Gender-related disparities in the frequencies of PD-1 and PD-L1 positive peripheral blood T and B lymphocytes in patients with alcohol-related liver disease: a single center pilot study

PeerJ

article

Beata Kasztelan-Szczerbinska¹  Katarzyna Adamczyk¹  Agata Surdacka²  Jacek Rolinski²  Agata Michalak¹  Agnieszka Bojarska-Junak²  Mariusz Szczerbinski³  Halina Cichoz-Lach¹ 

[1] Department of Gastroenterology with Endoscopy Unit, Medical University of Lublin;Department of Clinical Immunology, Medical University of Lublin;Department of Gastroenterology with Endoscopy Unit, Public, Academic Hospital No 4

关键词: Alcohol-related liver disease;    T lymphocytes;    B lymphocytes;    Programmed cell death-1 receptor;    Programmed cell death receptor ligand 1;   

DOI  :  10.7717/peerj.10518

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundExposure to excessive alcohol consumption dysregulates immune signaling. The programed cell death 1 (PD-1) receptor and its ligand PD-L1 play a critical role in the protection against immune-mediated tissue damage. The aim of our study was evaluation of the PD-1/PDL-1 expression on peripheral T and B lymphocytes, its correlation with markers of inflammation and the severity of liver dysfunction in the course of alcohol-related liver disease (ALD).Material and MethodsFifty-six inpatients with ALD (38 males, 18 females, aged 49.23 ± 10.66) were prospectively enrolled and assigned to subgroups based on their: (1) gender, (2) severity of liver dysfunction (Child-Pugh, MELD scores, mDF), (3) presence of ALD complications, and followed for 30 days. Twenty-five age- and gender-matched healthy volunteers served as the control group. Flow cytometric analysis of the PD-1/PD-L1 expression on peripheral lymphocyte subsets were performed.Results20. No correlations of PD-1+ and PD-L1+ lymphocyte percentages with mDF, CTP and MELD scores, nor with complications of ALD were observed. Significant correlations of PD-L1 positive B cell frequencies with conventional markers of inflammation were found.ConclusionsGender-related differences in the frequencies of PD-1/PD-L1 positive T and B cells were observed in patients with ALD. Upregulation of PD-1+/PD-L1+ lymphocytes paralleled both the severity of alcoholic hepatitis and liver dysfunction in ALD females.

【 授权许可】

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