期刊论文详细信息
PeerJ
Biomarker identification of isolated compartments of the cell wall, cytoplasm and vacuole from the internodal cell of characean Nitellopsis obtusa
article
Brigita Gylytė1  Sigita Jurkonienė1  Reda Cimmperman1  Vaidevutis Šveikauskas1  Levonas Manusadžianas1 
[1] Institute of Botany, Nature Research Centre
关键词: Biomarkers;    Cell compartments;    Cell wall;    Cytoplasm;    Vacuole;    Nitellopsis obtusa;    CuO nanoparticles;   
DOI  :  10.7717/peerj.10930
学科分类:社会科学、人文和艺术(综合)
来源: Inra
PDF
【 摘 要 】

Cells of characean algae are attractive for plant cell physiologists because of their large size and their close relation to higher plant cells. The objective of our study was to evaluate the purity of the compartments (cell wall, cytoplasm with plastids, mitochondria, nuclei and endomembrane system, and vacuole) separated mechanically from the internodal cells of Nitellopsis obtusa using enzymatic markers. These included α-mannosidase and malate dehydrogenase, vacuolar and cytoplasmic enzymes, respectively. The biomarkers applied revealed the degree of compartment contamination with the material from unwanted cell parts. The cell wall was contaminated slightly by vacuole and cytoplasm residuals, respectively by 12.3 and 1.96% of corresponding biomarker activities. Relatively high activity of vacuolar marker in the cell wall could be associated with the cell vacuoles in the multicellular structure of the nodes. The biomarkers confirmed highly purified vacuolar (99.5%) and cytoplasmic (86.7%) compartments. Purity estimation of the cell fractions enabled reevaluating nCuO related Cu concentrations in the compartments of charophyte cell. The internalisation of CuO nanoparticles in N. obtusa cell occurred already after 0.5h. In general, the approach seems to be useful for assessing the accumulation and distribution of various xenobiotics and/or metabolites within plant cell. All this justifies N.obtusa internodal cells as a model organism for modern studies in cell biology and nanotoxicology.

【 授权许可】

CC BY   

【 预 览 】
附件列表
Files Size Format View
RO202307100006571ZK.pdf 2581KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:0次