Genome-wide identification, classification, and expression analysis of the HSF gene family in pineapple ( Ananas comosus )

PeerJ

article

Lulu Wang¹  Yanhui Liu¹  Mengnan Chai¹  Huihuang Chen¹  Mohammad Aslam¹  Xiaoping Niu⁴  Yuan Qin¹  Hanyang Cai¹ 

[1] State Key Lab of Ecological Pest Control for Fujian and Taiwan Crops;Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education;Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, College of Life Sciences;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University

关键词: AcHSF;    Phylogenetic analysis;    Pineapple;    Cold;    Heat;    ABA;   

DOI  :  10.7717/peerj.11329

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Transcription factors (TFs), such as heat shock transcription factors (HSFs), usually play critical regulatory functions in plant development, growth, and response to environmental cues. However, no HSFs have been characterized in pineapple thus far. Here, we identified 22 AcHSF genes from the pineapple genome. Gene structure, motifs, and phylogenetic analysis showed that AcHSF families were distinctly grouped into three subfamilies (12 in Group A, seven in Group B, and four in Group C). The AcHSF promoters contained various cis-elements associated with stress, hormones, and plant development processes, for instance, STRE, WRKY, and ABRE binding sites. The majority of HSFs were expressed in diverse pineapple tissues and developmental stages. The expression of AcHSF-B4b/AcHSF-B4c and AcHSF-A7b/AcHSF-A1c were enriched in the ovules and fruits, respectively. Six genes (AcHSF-A1a , AcHSF-A2, AcHSF-A9a, AcHSF-B1a, AcHSF-B2a, and AcHSF-C1a) were transcriptionally modified by cold, heat, and ABA. Our results provide an overview and lay the foundation for future functional characterization of the pineapple HSF gene family.

【 授权许可】

CC BY