The correlation of WDR76 expression with survival outcomes and immune infiltrates in lung adenocarcinoma

PeerJ

article

Likui Fang¹  Guocan Yu¹  Wenfeng Yu¹  Gang Chen¹  Bo Ye¹ 

[1] Department of Thoracic Surgery, Affiliated Hangzhou Chest Hospital, Zhejiang University School of Medicine

关键词: Lung adenocarcinoma;    WDR76;    Prognosis;    Immune infiltrates;    TCGA;   

DOI  :  10.7717/peerj.12277

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundWD repeat domain 76 (WDR76) is a predicted member of the WD40-repeat-containing domain superfamily and possibly involves in various biological processes, but its function in cancers is poorly characterized. This study aimed to evaluate the role of WDR76 in the prognosis and immune infiltrates of lung adenocarcinoma (LUAD).MethodsWDR76 expressions in LUAD tissues and normal tissues were primarily compared by The Cancer Genome Atlas (TCGA) database, and were validated in cohorts from Gene Expression Omnibus (GEO) database. The associations between WDR76 expression and clinicopathologic characteristics were analyzed. Kaplan–Meier and Cox regression analyses were performed to determine the impact of WDR76 expression on survival outcomes. The protein interaction network of WDR76 was built using STRING website. TIMER and GEPIA databases were used to investigate the correlation between WDR76 expression and immune infiltrates.ResultsWDR76 expression was elevated in LUAD (P < 0.001) and high WDR76 expression was associated with advanced N stage, M stage and pathologic stage. Expectedly, high WDR76 expression significantly correlated with poor survival outcomes and was the independent risk factor for overall survival (OS) (HR 1.468, 95% CI [1.031–2.089], P = 0.033) and disease specific survival (DSS) (HR 1.764, 95% CI [1.095–2.842], P = 0.020). DDB1 and LSH were the important proteins interacting with WDR76. WDR76 expression correlated with CD8+ T cells presence and was also positively associated with levels of inhibitory receptors.ConclusionWDR76 expression was involved in the regulation of immune infiltrates and had predictive value for prognosis in LUAD.

【 授权许可】

CC BY