PODN is a prognostic biomarker and correlated with immune infiltrates in osteosarcoma

Cancer Cell International

Jun Wen¹  Zhao Feng Zhu¹  Lun Qing Zhu²  Feng Yao²  Fu Yong Zhang²  Yun Fang Zhen²  Quan Wen Yuan²  Zheng Zhang³  Guang Hao Su³  Xiao Dong Wang⁴ 

[1] Clinical Pediatric School of Soochow University, 215025, Su Zhou, Jiang Su, China;Department of Orthopedics, Children’s Hospital of Soochow University, 215025, Su Zhou, Jiang Su, China;Department of Orthopedics, Children’s Hospital of Soochow University, 215025, Su Zhou, Jiang Su, China;Clinical Pediatric Institute, Children’s Hospital of Soochow University, 215025, Su Zhou, Jiang Su, China;Department of Orthopedics, Children’s Hospital of Soochow University, 215025, Su Zhou, Jiang Su, China;Clinical Pediatric School of Soochow University, 215025, Su Zhou, Jiang Su, China;

关键词: Osteosarcoma;    PODN;    Diagnosis;    Immune infiltrates;    Prognosis;    Biomarker;   

DOI  :  10.1186/s12935-021-02086-5

来源: Springer

【 摘 要 】

BackgroundOsteosarcomawas the most common primary bone malignancy in children and adolescents. It was imperative to identify effective prognostic biomarkers for this cancer. This study was aimed to identify potential crucial genes of osteosarcoma by integrated bioinformatics analysis.MethodsIdentification of differentially expressed genes from public data gene expression profiles (GSE42352), functional and pathway enrichment analysis, protein–protein interaction (PPI) network construction and module analysis, Cox regression and survival analysis was conducted.ResultsTotally 17 co-differential genes were found to be differentially expressed. These genes were enriched in biological processes, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) pathway of inflammatory immune response. PPI network was constructed with 63 differentially expressed genes that co-existed between the test set and the validation set. The area under the receiver operating characteristic curve (AUC value) was 0.855, which indicated that the expression of PODN had a good diagnostic value for osteosarcoma. Furthermore, Cox regression and survival analysis revealed 5 genes were statistically significant.ConclusionsPODN was regarded as a potential biomarker for the diagnosis and prognosis of osteosarcoma, ACTA2, COL6A1, FAP, OLFML2B and COL6A3, can be used as potential prognostic indicators for osteosarcoma.

【 授权许可】

CC BY