Construction of a high-density linkage map and detection of sex-specific markers in Penaeus japonicus

PeerJ

article

Yaqun Zhang¹  Chuantao Zhang²  Na Yao¹  Jingxian Huang²  Xiangshan Sun²  Bingran Zhao²  Hengde Li¹ 

[1] Chinese Academy of Fishery Sciences;Xiaying Enhancement and Experiment Station, Chinese Academy of Fishery Sciences

关键词: Linkage map;    Sex-specific markers;    Penaeus japonicus;    Genotyping-by-sequencing;   

DOI  :  10.7717/peerj.12390

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Penaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism is observed in P. japonicus, in which females grow faster and larger than males; therefore, a unisexual female culture of P. japonicus could improve the efficiency of productivity. However, the genetic mechanisms underlying sex determination in P. japonicus are unclear. In this study, we constructed a high-density genetic linkage map of P. japonicus using genotyping-by-sequencing (GBS) technology in a full-sib family. The final map was 3,481.98 cM in length and contained 29,757 single nucleotide polymorphisms (SNPs). These SNPs were distributed on 41 sex-averaged linkage groups, with an average inter-marker distance of 0.123 cM. One haplotype, harboring five sex-specific SNPs, was detected in linkage group 1 (LG1), and its corresponding confidence interval ranged from 211.840 to 212.592 cM. Therefore, this high-density genetic linkage map will be informative for genome assembly and marker-assisted breeding, and the sex-linked SNPs will be helpful for further studies on molecular mechanisms of sex determination and unisexual culture of P. japonicus in the future.

【 授权许可】

CC BY