DNA barcoding and morphological identification of spiny lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus

PeerJ

article

Sachithra Amarin Hettiarachchi¹  Ji-Yeon Hyeon¹  Angka Mahardini¹  Hyung-Suk Kim⁵  Jun-Hwan Byun¹  Han-Jun Kim⁶  Jong-Gyun Jeong⁷  Jung-Kyu Yeo⁷  Shin-Kwon Kim⁸  Se-Jae Kim⁴  Youn-Seong Heo⁷  Jonathan Sathyadith¹  Do-Hyung Kang¹  Sung-Pyo Hur¹ 

[1] Jeju Marine Research Center, Korea Institute of Ocean Science and Technology;Department of Fisheries and Aquaculture, Faculty of Fisheries and Marine Sciences & Technology, University of Ruhuna;Department of Ocean Science, University of Science and Technology;Department of Biology, Jeju National University;Department of Kinesiology, Jeju National University;Marine Ecosystem Research Center, Korea Institute of Ocean Science & Technology;LED-Marine Biology Convergence Technology Research Center, Pukyong National University;Aquaculture Research Division, National Institute of Fisheries Science

关键词: Spiny lobsters;    DNA barcoding;    Panulirus japonicus;    Panulirus longipes;    Panulirus homarus homarus;    Panulirus stimpsoni;    Morphology;    Cytochrome oxidase I;    Jeju Island;    Phylogenetics;   

DOI  :  10.7717/peerj.12744

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

To date, 19 species of spiny lobsters from the genus Panulirus have been discovered, of which only P. japonicus, P. penicilatus, P. stimpsoni, and P. versicolor have been documented in South Korean waters. In this study, we aimed to identify and update the current list of spiny lobster species that inhabit South Korean waters based on the morphological features and the phylogenetic profile of cytochrome oxidase I (COI) of mitochondrial DNA (mtDNA). Spiny lobsters were collected from the southern and eastern coasts of Jeju Island, South Korea. Phylogenetic analyses were performed using neighbor-joining (NJ), maximum likelihood (ML), and Bayesian inference (BI) methods. The ML tree was used to determine the spiny lobster lineages, thereby clustering the 17 specimens collected in this study into clades A, B, C, and D, which were reciprocally monophyletic with P. japonicus, P. homarus homarus, P. longipes, and P. stimpsoni, respectively. These clades were also supported by morphological examinations. Interestingly, morphological variations, including the connected pleural and transverse groove at the third abdominal somite, were observed in four specimens that were genetically confirmed as P. japonicus. This finding is novel within the P. japonicus taxonomical reports. Additionally, this study updates the documentation of spiny lobsters inhabiting South Korean waters as P. longipes and P. homarus homarus were recorded for the first time in this region.

【 授权许可】

CC BY