DNA barcoding and morphological identification of spiny lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus

PeerJ

Shin-Kwon Kim¹  Se-Jae Kim²  Hyung-Suk Kim³  Jonathan Sathyadith⁴  Angka Mahardini⁴  Jun-Hwan Byun⁴  Ji-Yeon Hyeon⁴  Do-Hyung Kang⁴  Sung-Pyo Hur⁴  Sachithra Amarin Hettiarachchi⁴  Jong-Gyun Jeong⁵  Jung-Kyu Yeo⁵  Youn-Seong Heo⁵  Han-Jun Kim⁶ 

[1] Aquaculture Research Division, National Institute of Fisheries Science, Busan, Republic of Korea;Department of Biology, Jeju National University, Jeju, Republic of Korea;Department of Kinesiology, Jeju National University, Jeju, Republic of Korea;Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju,Republic of Korea;LED-Marine Biology Convergence Technology Research Center, Pukyong National University, Busan,Republic of Korea;Marine Ecosystem Research Center, Korea Institute of Ocean Science & Technology,Busan, Republic of Korea;

关键词: Spiny lobsters;    DNA barcoding;    Panulirus japonicus;    Panulirus longipes;    Panulirus homarus homarus;    Panulirus stimpsoni;   

DOI  :  10.7717/peerj.12744

来源: DOAJ

【 摘 要 】

To date, 19 species of spiny lobsters from the genus Panulirus have been discovered, of which only P. japonicus, P. penicilatus, P. stimpsoni, and P. versicolor have been documented in South Korean waters. In this study, we aimed to identify and update the current list of spiny lobster species that inhabit South Korean waters based on the morphological features and the phylogenetic profile of cytochrome oxidase I (COI) of mitochondrial DNA (mtDNA). Spiny lobsters were collected from the southern and eastern coasts of Jeju Island, South Korea. Phylogenetic analyses were performed using neighbor-joining (NJ), maximum likelihood (ML), and Bayesian inference (BI) methods. The ML tree was used to determine the spiny lobster lineages, thereby clustering the 17 specimens collected in this study into clades A, B, C, and D, which were reciprocally monophyletic with P. japonicus, P. homarus homarus, P. longipes, and P. stimpsoni, respectively. These clades were also supported by morphological examinations. Interestingly, morphological variations, including the connected pleural and transverse groove at the third abdominal somite, were observed in four specimens that were genetically confirmed as P. japonicus. This finding is novel within the P. japonicus taxonomical reports. Additionally, this study updates the documentation of spiny lobsters inhabiting South Korean waters as P. longipes and P. homarus homarus were recorded for the first time in this region.

【 授权许可】

Unknown