B-box containing protein 1 from Malus domestica (MdBBX1) is involved in the abiotic stress response

PeerJ

article

Yaqing Dai¹  Ying Lu¹  Zhou Zhou¹  Xiaoyun Wang¹  Hongjuan Ge³  Qinghua Sun¹ 

[1] College of Life Science, Shandong Agricultural University;Institute of Shandong River Wetlands;Qingdao Academy of Agricultural Science

关键词: MdBBX1;    Malus domestica;    Arabidopsis;    Abiotic stress;    ROS;   

DOI  :  10.7717/peerj.12852

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

B-box proteins (BBXs), which act as transcription factors, mainly regulate photomorphogenesis. However, the molecular functions underlying the activity of plant BBXs in response to abiotic stress remain largely unclear. In this investigation, we found that a BBX from Malus domestica (MdBBX1) was involved in the response to various abiotic stresses. The expression of MdBBX1 was significantly upregulated in response to abiotic stresses and abscisic acid (ABA). Recombinant MdBBX1 increased stress tolerance in Escherichia coli cells. In addition, overexpression of MdBBX1 in Arabidopsis decreased sensitivity to exogenous ABA, resulting in a germination rate and root length that were greater and longer, respectively, than those of wild-type (WT) plants. Moreover, the expression of ABI5 was decreased in MdBBX1-overexpressing lines under ABA treatment. After salt and drought treatments, compared with the WT plants, the MdBBX1 transgenic plants displayed enhanced tolerance and had a higher survival rate. Furthermore, under salt stress, increased proline (PRO) contents, decreased levels of malondialdehyde (MDA), increased activity of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX)) and decreased accumulation of reactive oxygen species (ROS) were observed in the MdBBX1-overexpressing plants. Overall, our results provide evidence that MdBBX1 might play a critical role in the regulation of abiotic stress tolerance by reducing the generation of ROS.

【 授权许可】

CC BY