Identification and analysis of sucrose synthase gene family associated with polysaccharide biosynthesis in Dendrobium catenatum by transcriptomic analysis

PeerJ

article

Min Jiang¹  Shangyun Li¹  Changling Zhao¹  Mingfu Zhao¹  Shaozhong Xu¹  Guosong Wen¹ 

[1] Research & Development Center for Heath Product, College of Agronomy and Biotechnology, Yunnan Agricultural University;Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Eco-Chongming ,(IEC), School of Life Sciences, Fudan University

关键词: SUS;    Mannose;    RNA-Seq;    Evolution;    Expression analysis;    Dendrobium catenatum;   

DOI  :  10.7717/peerj.13222

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundDendrobium catenatum is a valuable traditional medicinal herb with high commercial value. D. catenatum stems contain abundant polysaccharides which are one of the main bioactive components. However, although some genes related to the synthesis of the polysaccharides have been reported, more key genes need to be further elucidated.ResultsIn this study, the contents of polysaccharides and mannose in D. catenatum stems at four developmental stages were compared, and the stems’ transcriptomes were analyzed to explore the synthesis mechanism of the polysaccharides. Many genes involved in starch and sucrose metabolisms were identified by KEGG pathway analysis. Further analysis found that sucrose synthase (SUS; EC 2.4.1.13) gene maybe participated in the polysaccharide synthesis. Hence, we further investigated the genomic characteristics and evolution relationships of the SUS family in plants. The result suggested that the SUS gene of D. catenatum (DcSUS) had undergone the expansion characterized by tandem duplication which might be related to the enrichment of the polysaccharides in D. catenatum stems. Moreover, expression analyses of the DcSUS displayed significant divergent patterns in different tissues and could be divided into two main groups in the stems with four developmental stages.ConclusionIn general, our results revealed that DcSUS is likely involved in the metabolic process of the stem polysaccharides, providing crucial clues for exploiting the key genes associated with the polysaccharide synthesis.

【 授权许可】

CC BY