Identification of key differential genes in intimal hyperplasia induced by left carotid artery ligation

PeerJ

article

Lina Zhang¹  Jianjun Gu²  Sichuan Wang¹  Fuming He¹  Kaizheng Gong¹ 

[1] Department of Cardiology, The Affiliated Hospital of Yangzhou University, Yangzhou University;Department of Cardiology, Northern Jiangsu People’s Hospital, Yangzhou University

关键词: Intimal hyperplasia;    Transcriptome;    KEGG pathway;    Protein–protein interaction network;   

DOI  :  10.7717/peerj.13436

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Intimal hyperplasia is a common pathological process of restenosis following angioplasty, atherosclerosis, pulmonary hypertension, vein graft stenosis, and other proliferative diseases. This study aims to screen for potential novel gene targets and mechanisms related to vascular intimal hyperplasia through an integrated microarray analysis of the Gene Expression Omnibus Database (GEO) database. Material and Methods The gene expression profile of the GSE56143 dataset was downloaded from the Gene Expression Omnibus database. Functional enrichment analysis, protein-protein interaction (PPI) network analysis, and the transcription factor (TF)-target gene regulatory network were used to reveal the biological functions of differential genes (DEGs). Furthermore, the expression levels of the top 10 key DEGs were verified at the mRNA and protein level in the carotid artery 7 days after ligation. Results A total of 373 DEGs (199 upregulated DEGs and 174 downregulated DEGs) were screened. These DEGs were significantly enriched in biological processes, including immune system process, cell adhesion, and several pathways, which were mainly associated with cell adhesion molecules and the regulation of the actin cytoskeleton. The top 10 key DEGs (Ptprc, Fn1, Tyrobp, Emr1, Itgb2, Itgax, CD44, Ctss, Ly86, and Aif1) acted as key genes in the PPI network. The verification of these key DEGs at the mRNA and protein levels was consistent with the results of the above-mentioned bioinformatics analysis. Conclusion The present study identified key genes and pathways involved in intimal hyperplasia induced by carotid artery ligation. These results improved our understanding of the mechanisms underlying the development of intimal hyperplasia and provided candidate targets.

【 授权许可】

CC BY