Differential gene expression analysis after DAPK1 knockout in hepatocellular carcinoma cells

PeerJ

article

Yuanqi Li¹  Hui Huang¹  Huajun Yu¹  Ting Mo¹  Ting Wei¹  Guodan Li¹  Yufang Jia¹  Xiaoqin Huang¹  Mingjin Tu¹  Xiuwen Yan¹  Haitao Zhang¹ 

[1] Guangdong Medical University

关键词: Bioinformatics analysis;    DAPK1;    Differential gene;    Hepatocellular carcinoma;    Related gene;   

DOI  :  10.7717/peerj.13711

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundThe mechanism through which death-associated protein kinase 1 (DAPK1) causes hepatocellular carcinoma (HCC) progression remains unclear. In this study, we aimed to identify key proteins that were altered after DAPK1 knockout.MethodsStable DAPK1 knockout HCC cell lines were established, then the differentially expressed genes (DEGs) of HCC were screened using the NetworkAnalyst database and enriched using the Metascape software. Protein-protein interaction networks (PPIs) were analyzed and visualized using the STRING database expansion.ResultsIn total, 732 differentially expressed genes were identified, including 415 upregulated genes and 317 downregulated genes. Through Cytoscape software scoring, 10 pivotal genes were found to be closely related to changes in DAPK1 expression; Kininogen-1 (KNG1), Complement C3 (C3), Metalloproteinase inhibitor 1 (TIMP1), and Alpha-2-HS-glycoprotein (AHSG) were the most strongly associated with DAPK1 expression changes. Moreover, western blot analysis results revealed that changes in the levels of proteins encoded by the four key genes after DAPK1 knockout were consistent with those seen in the database screening.ConclusionsThese results provide a direction for further studies on the DAPK1 gene and on the mechanism through which DAPK1 leads to hepatocellular carcinoma development.

【 授权许可】

CC BY