Genome-wide characterization and expression analysis of bHLH gene family in physic nut ( Jatropha curcas L.)

PeerJ

article

Lin Zhang¹  Wei Chen¹  Rongrong Liu¹  Ben Shi¹  Youju Shu¹  Haoyu Zhang¹ 

[1] School of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology

关键词: Physic nut;    bHLH transcription factor;    Abiotic stress;   

DOI  :  10.7717/peerj.13786

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

The basic helix loop helix (bHLH) transcription factor perform essential roles in plant development and abiotic stress. Here, a total of 122 bHLH family members were identified from the physic nut (Jatropha curcas L.) genomic database. Chromosomal localization results showed that 120 members were located on 11 chromosomes. The phylogenetic tree manifested that the JcbHLHs could be grouped into 28 subfamilies. Syntenic analysis showed that there were 10 bHLH collinear genes among the physic nut, Arabidopsis thaliana and Oryza sativa. These genes, except JcbHLH84, were highly expressed in various tissues of the physic nut, implying a key role in plant development. Gene expression profiles showed that ten genes (especially JcbHLH33, JcbHLH45 and JcbHLH55) correspond to both salinity and drought stresses; while eight genes only respond to salinity and another eight genes only respond to drought stress. Moreover, the protein interaction network revealed that the JcbHLHs are involved in growth, development and stress signal transduction pathways. These discoveries will help to excavate several key genes may involve in salt or drought stresses and seed development, elucidate the complex transcriptional regulation mechanism of JcbHLH genes and provide the theoretical basis for stress response and genetic improvement of physic nut.

【 授权许可】

CC BY