Targeted metabolomic profiles of serum amino acids and acylcarnitines related to gastric cancer

PeerJ

article

Dehong Li¹  Yan Lu²  Fenghui Zhao³  Li Yan²  Xingwen Yang²  Lianhua Wei²  Xiaoyan Yang²  Xiumei Yuan²  Kehu Yang¹ 

[1] Evidence Based Medicine Center, School of Basic Medical Sciences, Lanzhou University;Department of Clinical laboratory, Gansu Provincial Hospital;Department of Pathology, Gansu Provincial Hospital

关键词: Gastric cancer;    Atrophic Gastritis;    Superficial gastritis;    Amino acids;    Acylcarnitines;    Metabolomics;   

DOI  :  10.7717/peerj.14115

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundEarly diagnosis and treatment are imperative for improving survival in gastric cancer (GC). This work aimed to assess the ability of human serum amino acid and acylcarnitine profiles in distinguishing GC cases from atrophic gastritis (AG) and control superficial gastritis (SG) patients.MethodsSixty-nine GC, seventy-four AG and seventy-two SG control patients treated from May 2018 to May 2019 in Gansu Provincial Hospitalwere included. The levels of 42 serum metabolites in the GC, AG and SG groups were detected by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Then, orthogonal partial least squares discriminant analysis (OPLS-DA) and the Kruskal-Wallis H test were used to identify a metabolomic signature among the three groups. Metabolites with highest significance were examined for further validation. Receiver operating characteristic (ROC) curve analysis was carried out for evaluating diagnostic utility.Results1) and could distinguish GC patients from AG and SG cases. In comparison with the AG and SG groups, GC cases had significantly higher C6DC, C16OH, C6, C0 and ARG amounts. Jointly quantitating these five metabolites had specificity and sensitivity in GC diagnosis of 98.55% and 99.32%, respectively, with an area under the ROC curve (AUC) of 0.9977.ConclusionThis study indicates C6DC, C16OH, C6, C0 and ARG could effectively differentiate GC cases from AG and SG patients, and may jointly serve as a valuable circulating multi-marker panel for GC detection.

【 授权许可】

CC BY