Rapid discrimination of the native medicinal plant Adenostemma lavenia from its adulterants using PCR-RFLP

PeerJ

article

Kunchang Wu¹  Yunchen Liu¹  Bocheng Yang²  Yenying Kung³  Kaiwei Chang³  Mengshiou Lee² 

[1] School of Pharmacy, College of Pharmacy, China Medical University;Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University;Institute of Traditional Medicine, College of Medicine, National Yang Ming Chiao Tung University;Center of Traditional Medicine, Taipei Veterans General Hospital;Faculty of Medicine, School of Medicine, National Yang Ming Chiao Tung University

关键词: Adenostemma lavenia;    Identification of origin;    Molecular identification;    Functional food;   

DOI  :  10.7717/peerj.13924

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundIn Taiwan, the aerial part of Adenostemma lavenia (Al) is used in the form of herbal tea or in a folk remedy primarily to mitigate inflammatory conditions in the lungs and liver. Due to the excellent health benefits of Al against inflammation, it has become increasingly crucial and in great demand during the COVID-19 pandemic. However, Al has been found to be adulterated with Wedelia biflora, Sigesbeckia orientalis, and/or Wedelia chinensis because of similarities in appearance and vernacular names.MethodsThis study aimed to develop a PCR-RFLP DNA molecular method for the authentication of Al. The restriction enzyme BsrI was used according to the sequencing and alignment results of PCR products in the ITS2 regions of Al and its adulterants. Gel electrophoresis resulted in the clear separation of Al and its adulterants into two distinct categories.ResultsIn conclusion, the PCR-RFLP authentication method developed herein provides an easy, rapid, and accurate method to distinguish Al from its adulterants to assure user health and safety.

【 授权许可】

CC BY