Identification and expression analysis of maize NF-YA subunit genes

PeerJ

article

Mingyue Lv¹  Hongzhe Cao¹  Xue Wang²  Kang Zhang¹  Helong Si¹  Jinping Zang¹  Jihong Xing¹  Jingao Dong¹ 

[1] State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultrual University;Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University

关键词: Maize;    NF-YA subunit gene;    Biological stress;    Abiotic stress;    Expression analysis;   

DOI  :  10.7717/peerj.14306

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

NF-YAs encode subunits of the nuclear factor-Y (NF-Y) gene family. NF-YAs represent a kind of conservative transcription factor in plants and are involved in plant growth and development, as well as resistance to biotic and abiotic stress. In this study, 16 maize (Zea mays) NF-YA subunit genes were identified using bioinformatics methods, and they were divided into three categories by a phylogenetic analysis. A conserved domain analysis showed that most contained a CCAAT-binding transcription factor (CBFB) _NF-YA domain. Maize NF-YA subunit genes showed very obvious tissue expression characteristics. The expression level of the NF-YA subunit genes significantly changed under different abiotic stresses, including Fusarium graminearum infection and salicylic acid (SA) or jasmonic acid (JA) treatments. After inoculation with Setosphaeria turcica and Cochliobolus heterostrophus, the lesion areas of nfya01 and nfya06 were significantly larger than that of B73, indicating that ZmNFYA01 and ZmNFYA06 positively regulated maize disease resistance. ZmNFYA01 and ZmNFYA06 may regulated maize disease resistance by affecting the transcription levels of ZmPRs. Thus, NF-YA subunit genes played important roles in promoting maize growth and development and resistance to stress. The results laid a foundation for clarifying the functions and regulatory mechanisms of NF-YA subunit genes in maize.

【 授权许可】

CC BY