Morphological, ultrastructural and molecular variations in susceptible and resistant genotypes of chickpea infected with Botrytis grey mould

PeerJ

article

Richa Thakur¹  Rajni Devi²  Milan Kumar Lal³  Rahul Kumar Tiwari⁴  Sucheta Sharma¹  Ravinder Kumar⁴ 

[1] Department of Biochemistry, Punjab Agricultural University;Department of Microbiology, Punjab Agricultural University;Division of Crop Physiology, Biochemistry and Post harvest Technology, ICAR-Central Potato Research Institute;Division of Plant Protection, ICAR-Central Potato Research Institute

关键词: Botrytis cinerea;    Chickpea;    Leison;    Inoculation;    Resistance;   

DOI  :  10.7717/peerj.15134

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Biotic stress due to fungal infection is detrimental to the growth and development of chickpea. In our study, two chickpea genotypes viz Cicer pinnatifidum (resistant) and PBG5 (susceptible) were inoculated with (1 × 104 spore mL−1) of nectrotrophic fungus Botrytis cinerea at seedling stage. These seedlings were evaluated for morphological, ultrastructural, and molecular differences after 3, 5 and 7 days post inoculation (dpi). Visual symptoms were recorded in terms of water-soaked lesions, rotten pods and twigs with fungal colonies. Light and scanning electron microscopy (SEM) revealed the differences in number of stomata, hyphal network and extent of topographical damage in resistant (C. pinnatifidum) and susceptible (PBG5) genotypes, which were validated by stomatal index studies done by using fluorescence microscopy in the infection process of B. cinerea in leaves of both chickpea genotypes. In case of control (water inoculated) samples, there were differences in PCR analysis done using five primers for screening the genetic variations between two genotypes. The presence of a Botrytis responsive gene (LrWRKY) of size ~300 bp was observed in uninoculated resistant genotype which might have a role in resistance against Botrytis grey mould. The present investigation provides information about the variation in the infection process of B. cinerea in two genotypes which can be further exploited to develop robust and effective strategies to manage grey mould disease.

【 授权许可】

CC BY