Microsatellite markers-aided dissection of iron, zinc and cadmium accumulation potential in Triticum aestivum

PeerJ

article

Asima Rasheed¹  Javed Ahmad²  Majid Nadeem²  Muhammad Abdul Rehman Rashid¹  Farrukh Azeem¹ 

[1]Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad

[2]Wheat Research Institute, Ayub Agricultural Research Institute ,(AARI), Faisalabad

关键词: Wheat;    Biofortification;    Iron;    Zinc;    Cadmium;    SSR markers;    Marker assisted selection;    Bioavailability;    Gene specific primers;    Tassel;   

DOI  :  10.7717/peerj.15229

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Background Wheat is a staple cereal food around the globe. It provides a significant source of proteins, carbohydrates, and other micronutrients to humans. When grown on cadmium (Cd) contaminated soils, the uptake of trace elements e.g., iron (Fe) and zinc (Zn) has also been affected drastically that in turn affected the wheat grain. Methods In this study, wheat accessions were used to investigate the impact of soil application of Zn (5 mg/kg, 20 mg/kg) and Cd (0 mg/kg, 10 mg/kg) on accumulation of these elements in wheat grains. A total of 45 Fe, Zn, and Cd transporter-related genes were used to design 101 gene-specific SSR (simple sequence repeat) markers. Results In response to Cd stress, application of 20 mg/Kg Zn improved Fe (64.6 ug/g) and Zn (48.3 ug/g) accumulation in wheat grains as well as agronomic traits. Marker trait association revealed that SSR markers based on NAM-B1 gene (PR01 and PR02) were associated with Zn accumulation. Similarly, SSR markers based on TaVTL5-2B_5 (PR19 PR20), TaVTL5-2B_2 (PR25, PR26), TaVTL5-2D_3 (PR30), TaVTL2-2A (PR31), TaVTL1-6A (PR32), and TaVTL2-2D_1 (PR37) were significantly associated with Fe accumulation, while HMA3-5B1 (PR62) and TaNRAMP3-7D (PR89) were linked to Cd accumulation in grains. The highly associated markers may be used in marker-assisted selection of suitable wheat genotypes for breeding bio-fortified varieties with low Cd accumulation.

【 授权许可】

CC BY