Transcriptomic and metabolomic analysis of autumn leaf color change in Fraxinus angustifolia

PeerJ

article

Yanlong Wang¹  Jinpeng Zhen²  Xiaoyu Che¹  Kang Zhang²  Guowei Zhang³  Huijuan Yang¹  Jing Wen¹  Jinxin Wang¹  Jiming Wang¹  Bo He⁵  Ailong Yu⁶  Yanhui Li¹  Zhigang Wang¹ 

[1] College of Landscape Architecture and Tourism, Hebei Agricultural University;Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Bioinformatics Utilization and Technological Innovation Center for Agricultural Microbes, Hebei Agricultural University;Hongyashan State-owned Forest Farm in Hebei Province;College of Grammar, Hebei Normal University of Science and Technology;Green Building Development Center of Baoding;Flower and Wood Technical Service Center of Hengshui

关键词: Fraxinus angustifolia;    Transcriptomes;    Metabolomes;    Anthocyanin;    Gene expression;   

DOI  :  10.7717/peerj.15319

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Fraxinus angustifolia is a type of street tree and shade tree with ornamental value. It has a beautiful shape and yellow or reddish purple autumn leaves, but its leaf color formation mechanism and molecular regulation network need to be studied. In this study, we integrated the metabolomes and transcriptomes of stage 1 (green leaf) and stage 2 (red-purple leaf) leaves at two different developmental stages to screen differential candidate genes and metabolites related to leaf color variation. The results of stage 1 and stage 2 transcriptome analysis showed that a total of 5,827 genes were differentially expressed, including 2,249 upregulated genes and 3,578 downregulated genes. Through functional enrichment analysis of differentially expressed genes, we found that they were involved in flavonoid biosynthesis, phenylpropanoid biosynthesis, pigment metabolism, carotene metabolism, terpenoid biosynthesis, secondary metabolite biosynthesis, pigment accumulation, and other biological processes. By measuring the metabolites of Fraxinus angustifolia leaves, we found the metabolites closely related to the differentially expressed genes in two different periods of Fraxinus angustifolia, among which flavonoid compounds were the main differential metabolites. Through transcriptome and metabolomics data association analysis, we screened nine differentially expressed genes related to anthocyanins. Transcriptome and qRT-PCR results showed that these nine genes showed significant expression differences in different stages of the sample, and we speculate that they are likely to be the main regulatory factors in the molecular mechanism of leaf coloration. This is the first time that we have analyzed the transcriptome combination metabolome in the process of leaf coloration of Fraxinus angustifolia, which has important guiding significance for directional breeding of colored-leaf Fraxinus species and will also give new insights for enriching the landscape.

【 授权许可】

CC BY