Vitamin E supplementation may negatively affect preimplantation development and mitochondrial ultrastructure of vitrified murine embryos

Asian Pacific Journal of Reproduction

article

Sarbandi, Mimi-Sophia¹  Noor Khan, Nor-Ashikin Mohamed Noor¹  Rahman, Nor-Shahida Abdul¹  Eshak, Zolkapli⁴  Abdullah, Fathiah¹  Malek, Mastura Abd¹  Kamal, Aqila-Akmal Mohammad¹ 

[1] Maternofetal and Embryo Research Group, Faculty of Medicine, Universiti Teknologi MARA, Selangor Branch, Sungai Buloh Campus;Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, Tapah Campus;Faculty of Health Sciences, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus;Faculty of Pharmacy, Universiti Teknologi MARA, Selangor Branch, Puncak Alam Campus

关键词: Mitochondria;    Preimplantation;    Embryo;    Vitrification;    Vitamin E;    Alpha-tocopherol;    Tocotrienol-rich-fraction;    Transmission electron microscopy;   

DOI  :  10.4103/2305-0500.316626

学科分类：兽医学

来源: Wolters Kluwer Medknow Publications

【 摘 要 】

Objective:  To observe the effects of vitamin E on post-vitrification preimplantation development, gross morphology as well as mitochondrial distribution and ultrastructure. Methods:  Twenty-four female C57BL/6NTac mice, aged 12-16 weeks, were randomly divided into four groups. Group A did not receive any treatment and served as the control group. Group B was treated with corn oil stripped of tocopherols and served as the vehicle group. Group C was treated with 60 mg/kg body weight of tocotrienol-rich-fraction with corn oil stripped of tocopherols. Group D was treated with 60 mg/kg body weight of alpha-tocopherol with corn oil stripped of tocopherols. All treatments were administered orally for 7 consecutive days. After superovulation and mating with fertile males, 2-cell stage embryos were harvested for vitrification. Post vitrification development in vitro, gross morphology and ultrastructure were compared between groups. Results:  The number of 2 and 8-cell embryo, and blastocysts in the treatment and control groups were not significantly different (P0.05). Following vitrification, all 2-cell embryos had equal-sized blastomeres and intact zona pellucida. Mitochondrial aggregation toward the perinuclear region was seen in all of the treatment groups. Both groups C and D had vacuolated mitochondria, which was reflected in the trend of preimplantation development reduction. Conclusions:  Vitamin E supplementation of 60 mg/kg body weight does not improve the viability of healthy embryos according to this study. As a result, the most effective dose of vitamin E supplementation may be determined by the initial quality of the embryos.

【 授权许可】

CC BY-NC-SA