Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801

The Korean Journal of Parasitology

article

Shasha Gong¹  Qingling Meng¹  Jun Qiao¹  Yunfu Huang¹  Wenqiang Zhong¹  Guowu Zhang¹  Kai Zhang¹  Ningxing Li¹  Yunxia Shang¹  Zhiyuan Li¹  Xuepeng Cai² 

[1] College of Animal Science & Technology, Shihezi University;State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

关键词: Arthrobotrys oligospora;    chitinase;    enzyme characteristic;    biological activity;   

DOI  :  10.3347/kjp.2022.60.5.345

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)8 triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40°C and pH values between 4–7. Enzyme activity was inhibited by Zn2+, Ca2+, and Fe3+, whereas Mg2+ and K+ potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.

【 授权许可】

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