Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus

Sociedade Brasileira de Medicina Tropical. Revista

article

Fernandes, Blenda¹  Santrer, Emanoelle Fernandes Rutren La¹  Figueiredo, Sônia Maria de²  Rocha-Silva, Fabiana¹  Assunção, Cláudia Barbosa¹  Abreu, Amanda Gabrielle¹  Santiago, Iara Furtado³  Johann, Susana³  Caligiorne, Rachel Basques¹ 

[1] Hospital Santa Casa de Belo Horizonte;Universidade Federal de Ouro Preto;Universidade Federal de Minas Gerais

关键词: Sporothrix sp;    Polymerase chain reaction;    Sporotrichosis;    Molecular diagnosis;   

DOI  :  10.1590/0037-8682-0217-2022

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Background:Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. Methods: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. Results: Three specific primers with 100% specificity for the Sporothrix genus were generated. Conclusions: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.

【 授权许可】

CC BY