Molecular characterization of Salmonella strains in individuals with acute diarrhea syndrome in the State of Sucre, Venezuela

Revista da Sociedade Brasileira de Medicina Tropical

Hectorina Rodulfo²  Marcos De Donato²  Jesús Luiggi²  Elvia Michelli¹  Adriana Millán²  Miriam Michelli² 

[1] ,Universidade de Oriente Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas Dra. Suzan Tai Laboratório de Genética MolecularCumaná Sucre ,Venezuela

关键词: Molecular diagnosis;    Coproculture;    Acute diarrhea;    Polymerase chain reaction;    Salmonella;    Diagnóstico molecular;    Coprocultura;    Diarreia aguda;    Reação em cadeia da polimerase;    Salmonella;   

DOI  :  10.1590/S0037-86822012000300010

来源: SciELO

【 摘 要 】

INTRODUCTION:In Venezuela, acute diarrheic syndrome (ADS) is a primary cause of morbi-mortality, often involving the Salmonella genus. Salmonella infections are associated with acute gastroenteritis, one of the most common alimentary intoxications, and caused by the consumption of contaminated water and food, especially meat. METHODS: Conventional and molecular methods were used to detect Salmonella strains from 330 fecal samples from individuals of different ages and both sexes with ADS. Polymerase chain reaction (PCR) was used for the molecular characterization of Salmonella, using invA, sefA, and fliC genes for the identification of this genus and the serotypes Enteritidis and Typhimurium, respectively. RESULTS: The highest frequency of individuals with ADS was found in children 0-2 years old (39.4%), and the overall frequency of positive coprocultures was 76.9%. A total of 14 (4.2%) strains were biochemically and immunologically identified as Salmonella enterica subsp. enterica, of which 7 were classified as belonging to the Enteritidis serotype, 4 to the Typhimurium serotype, and 3 to other serotypes. The S. enterica strains were distributed more frequently in the age groups 3-4 and 9-10 years old. CONCLUSIONS: The molecular characterization method used proved to be highly specific for the typing of S. enterica strains using DNA extracted from both the isolated colonies and selective enrichment broths directly inoculated with fecal samples, thus representing a complementary tool for the detection and identification of ADS-causing bacteria.

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