Gene Ontology Assessment of Indirect Cold Physical Plasma and UV-Radiation Molecular Mechanism at the Cellular Level

Journal of Lasers in Medical Sciences

article

ZahraRazzaghi¹  BabakArjmand²  MaryamHamzeloo Moghadam³  Mostafa Rezaei-Tavirani⁴ 

[1] Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences;Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences;Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences;Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences

关键词: Chemical pathway analysis;    cold physical plasma;    UV-radiation;    gene expression change;    gene ontology;   

DOI  :  10.34172/jlms.2023.10

学科分类：基础医学

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Introduction: The development of therapeutic methods implies an understanding of the molecular mechanism of the applied methods. Due to the widespread use of UV radiation and cold physical plasma in medicine, the molecular mechanism of these two methods is compared via gene ontology.Methods: Data were derived from Gene Expression Omnibus (GEO). The differentially expressed genes (DEGs) which discriminate the cells treated with UV radiation versus indirect cold physical plasma were analyzed via gen ontology enrichment. The related biochemical pathways were extracted from the “Kyoto Encyclopedia of Genes and Genomes” (KEGG).Results: Among the 152 queried DEGs, 18 critical genes including SOC1, LDLR, ALO5, PTGS2, TNF, JUNB, TNFRSF1A, CD40, SMAD7, ID1, SMAD6, SERPINE1, PMAIP1, MDM2, CREB5, GADD45A, E2F3, and ETV5 were highlighted as the genes that victimize the two methods.Conclusion: NOTCH1 and TNF as the main genes plus SEREPINE1, KLF, and BDNF were introduced as the significant genes that are involved in the processes which discriminate cold physical plasma administration and UV-radiation as the two evaluated therapeutic methods.

【 授权许可】

CC BY