Insight into the Impact of Oxidative Stress on the Barrier Properties of Lipid Bilayer Models

International Journal of Molecular Sciences

Thomas von Woedtke¹  Mehdi Ravandeh¹  Johanna Striesow¹  Zahra Nasri¹  Kristian Wende¹  Mohsen Ahmadi¹ 

[1] Center for Innovation Competence Plasmatis, Leibniz Institute for Plasma Science and Technology, 17489 Greifswald, Germany;

关键词: reactive oxygen and nitrogen species;    cold physical plasma;    cell membrane;    supported lipid bilayers;    electrochemistry;    Langmuir-Blodgett and Schaefer techniques;   

DOI  :  10.3390/ijms23115932

来源: DOAJ

【 摘 要 】

As a new field of oxidative stress-based therapy, cold physical plasma is a promising tool for several biomedical applications due to its potential to create a broad diversity of reactive oxygen and nitrogen species (RONS). Although proposed, the impact of plasma-derived RONS on the cell membrane lipids and properties is not fully understood. For this purpose, the changes in the lipid bilayer functionality under oxidative stress generated by an argon plasma jet (kINPen) were investigated by electrochemical techniques. In addition, liquid chromatography-tandem mass spectrometry was employed to analyze the plasma-induced modifications on the model lipids. Various asymmetric bilayers mimicking the structure and properties of the erythrocyte cell membrane were transferred onto a gold electrode surface by Langmuir-Blodgett/Langmuir-Schaefer deposition techniques. A strong impact of cholesterol on membrane permeabilization by plasma-derived species was revealed. Moreover, the maintenance of the barrier properties is influenced by the chemical composition of the head group. Mainly the head group size and its hydrogen bonding capacities are relevant, and phosphatidylcholines are significantly more susceptible than phosphatidylserines and other lipid classes, underlining the high relevance of this lipid class in membrane dynamics and cell physiology.

【 授权许可】

Unknown