Optogenetic Stimulation of Primary Cardiomyocytes Expressing ChR2

Journal of Lasers in Medical Sciences

article

Hoda Keshmiri Neghab¹  Mohammad Hasan Soheilifar³  Ali Akbar Saboury²  Bahram Goliaei²  Jun Hong⁵  Gholamreza Esmaeeli Djavid¹ 

[1] Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute;Institutes of Biochemistry and Biophysics ,(IBB), University of Tehran;Department of Medical Laser, Medical Laser Reserch Center, Yara Institute;Research Center for Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences;School of Life Sciences, Henan University

关键词: Primary cardiomyocyte;    Optogenetics;    ChR2;   

DOI  :  10.34172/jlms.2021.32

学科分类：基础医学

来源: Shahid Beheshti University of Medical Sciences

【 摘 要 】

Introduction: Non-clinical cardiovascular drug safety assessment is the main step in the progress of new pharmaceutical products. Cardiac drug safety testing focuses on a delayed rectifier potassium channel block and QT interval prolongation, whereas optogenetics is a powerful technology for modulating the electrophysiological properties of excitable cells.Methods: For this purpose, the blue light-gated ion channel, channelrhodopsin-2 (ChR2), has been introduced into isolated primary neonatal cardiomyocytes via a lentiviral vector. After being subjected to optical stimulation, transmembrane potential and intracellular calcium were assessed.Results: Here, we generated cardiomyocytes expressing ChR2 (light-sensitive protein), that upon optical stimulation, the cardiomyocytes depolarized result from alterations of membrane voltage and intracellular calcium.Conclusion: This cell model was easily adapted to a cell culture system in a laboratory, making this method very attractive for therapeutic research on cardiac optogenetics.

【 授权许可】

CC BY