Using light to control and investigate cell biology

Optogenetics;Cell Signaling;Carbon Dots

Khamo, John S.

关键词: Optogenetics;    Cell Signaling;    Carbon Dots;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/105591/KHAMO-DISSERTATION-2019.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

It is no surprise that light plays an important role in life. With the development of modern technologies, light has also proven to be a powerful tool for the interrogation of molecular and cellular biology. Beyond its use in microscopic applications, light has emerged as an attractive modulator of cellular function. Within the last two decades, scientists have begun to harness the power of light and light-sensitive moieties to synthetically alter molecular processes in living cells. In combination with more conventional experimental techniques, this approach has allowed for the precise elucidation of many biological mechanisms that underpin development and disease. Here, I report on recent advances in optobiological techniques and their application in cell biology. I also include a novel study on the optogenetic delineation of growth factor signaling in neuronal development. Finally, I report on the spectroscopic analysis of fluorescent nanoparticles with potential biological utility.