Determination of Coumarin in Mikania glomerata Infusions by Square-Wave Voltammetry Using a Boron-Doped Diamond Electrode

Journal of the Brazilian Chemical Society

article

Nóbrega, Denise M.¹  Lourenço, Anabel S.¹  Fernandes, David Douglas S.¹  Lyra, Wellington S.¹  Moreira, Edilene D. T.¹  Diniz, Paulo Henrique G. D.²  Araújo, Mario C. U.¹ 

[1] Universidade Federal da Paraíba;Universidade Federal do Oeste da Bahia

关键词: coumarin;    square wave voltammetry;    boron-doped diamond electrode;   

DOI  :  10.21577/0103-5053.20220152

学科分类：内科医学

来源: SciELO

【 摘 要 】

Infusions of Mikania glomerata Sprengel (guaco) leaves are used in Brazil for the treatment of respiratory diseases. Coumarin is the chemical marker of guaco for quality control purposes. This work proposes a voltammetric methodology to determine coumarin by using a boron-doped diamond electrode. At pH 10 (0.1 mol L-1 Britton-Robinson buffer solution), coumarin is irreversibly oxidized around +1.77 V in a process predominantly controlled by diffusion. The methodology described here presented a linear range from 1.54 to 15.3 µg mL-1 with limits of detection and quantification of 0.20 and 0.62 µg mL-1, respectively. Analysis of variance confirmed the significance of the regression and the absence of lack-of-fit at the confidence level of 95%. Infusion of commercial guaco leaves was directly analyzed, and results were in agreement with high-performance liquid chromatography after applying the paired t-test at a confidence level of 95%.

【 授权许可】

CC BY