Voltammetric Determination of Ethionamide in Pharmaceutical Formulations and Human Urine using a Boron-Doped Diamond Electrode

Journal of the Brazilian Chemical Society

Batista, Bruna L.¹  Leite, Fernando R. F.¹  Ferraz, Bruno R. L.¹  Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brazil¹  Malagutti, Andréa R.¹ 

关键词:  ethionamide;    square wave voltammetry;    boron-doped diamond electrode;    antitubercular drugs;   

DOI  :  10.5935/0103-5053.20150313

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

This work reports a simple and low cost voltammetric approach comprising a boron-doped diamond electrode (BDDE) to determine the antibiotic ethionamide (ETO). Cyclic voltammetry studies revealed that ETO exhibits an irreversible reduction peak at -0.95 V and an irreversible oxidation peak at +1.4 V onto BDDE (vs. saturated Ag/AgCl reference electrode) in Britton-Robinson buffer solution (pH 5.0, 0.1 mol L-1). Different voltammetric scan rates (from 10 to 150 mV s-1) suggested that the reduction of ETO on the BDDE surface is a diffusion-controlled process. Square wave voltammetry (SWV) optimized conditions showed a linear response to ETO from 1.00 to 80.0 µmol L-1 (R2 = 0.998) with a limit of detection of 0.294 µmol L-1 and limit of quantification of 0.980 µmol L-1. The developed square wave voltammetric method was successfully used in the determination of ETO in human urine and pharmaceutical formulation. The ETO quantification results in pharmaceutical tablets obtained by SWV-BDDE were comparable to those found by official analytical protocols.

【 授权许可】

Unknown