Artificial Diuresis: Animal Studies on Efficacy and Safety of a New Miniaturized Device for Extracorporeal Ultrafiltration

Cardiorenal medicine

article

Lorenzin, Anna¹  Sgarabotto, Luca¹  Bacci, Maria Laura³  Elmi, Alberto³  Ventrella, Domenico³  Aniballi, Camilla³  Zanella, Monica¹  Brendolan, Alessandra¹  Di Lullo, Luca⁴  Ronco, Claudio² 

[1] Department of Nephrology Dialysis and Transplant St. Bortolo Hospital;International Renal Research Institute of Vicenza;Department of Veterinary Medical Science, Bologna University;Department of Nephrology

关键词: Ultrafiltration;    Animal study;    Artificial Diuresis-1;    Extracorporeal therapy;    Fluid overload;    Miniaturized extracorporeal device;   

DOI  :  10.1159/000530382

学科分类：心脏病和心血管学

来源: S Karger AG

【 摘 要 】

Introduction: We have recently developed a new miniaturized device for extracorporeal ultrafiltration (UF) to be used in patients with fluid overload: Artificial Diuresis-1 (AD1) (Medica S.p.A., Medolla, Italy). The device has a reduced priming volume, operates at very low pressures and flow regimes, and is designed to perform extracorporeal UF at bedside. After accurate experiments were carried out in vitro, we report in this paper the results of in vivo UF sessions carried out in selected animals according to veterinary best practice. Materials and Methods: The AD1 kit is pre-filled with sterile isotonic solution and operates with a polysulfone mini-filter, MediSulfone (polysulfone at 50,000 Dalton). A collection bag with a volumetric scale is connected to the UF line, and the ultrafiltrate is obtained by gravity based on the height at which the ultrafiltrate collection bag is placed. Animals were prepared and anesthetized. The jugular vein was cannulated with a double-lumen catheter. Three 6-h sessions of UF were scheduled with a target fluid removal of 1,500 mL. Heparin was used as anticoagulant. Results: In all treatments, the target value of UF was obtained in the absence of major clinical or technical problems with a maximum deviation from the scheduled UF rate lower than 10%. The device resulted to be safe, reliable, accurate, and easily usable thanks to a user-friendly interface and its very small dimensions. Conclusions: This study opens the way for clinical trials in different settings including departments with low intensity of care and even in ambulatory centers or patient’s home.

【 授权许可】

CC BY