Investigation Of STAT5A, OLR1, LF And TLR4 Variants And Its Association With Milk Yield In Egyptian Buffalo.

Research Journal of Pharmaceutical, Biological and Chemical Sciences

article

Nermin El-Halawany¹  Abd-El-Monsif A. Shawky¹  Sherif M. Sherif²  Afaf E. M. El-Wardany¹  Magdy A. Abdel-Latif³  Hamdy Abdel-Shafy⁴  Ahmed F.M. Al-Tohamy¹  Yasmin El-Sayed.¹ 

[1] Cell Biology Department, National Research Centre;Department of Chemistry, Faculty of science, Cairo University;Department of Buffalo Research, Animal Production Research Institute;Department of Animal Production, Faculty of Agriculture, Cairo University

关键词: Buffalo;    milk yield;    STAT5A;    OLR1;    LF;    TLR4;    Sequenom assay.;   

DOI  :  10.33887/rjpbcs/2019.10.3.23

学科分类：工程和技术（综合）

来源: Research Journal of Pharmaceutical, Biological and Chemical Sciences

【 摘 要 】

In this work, we selected 19 SNPs that are located in 4 genes, STAT5A, OLR1, LF and TLR4, because oftheir contribution and association with milk production traits in cattle. The selected SNPs were genotyped on 74multiparous Egyptian buffaloes via sequenom assay. Only 7 SNPs showed polymorphic patterns, one of them islocated in intron 1 of OLR1 gene at nucleotide position 423. This SNP found to have a minor allele frequency(MAF) of 0.14. The other one lies in intron 1 of TLR4 at nucleotide position 4525, its MAF is 0.025. The remaining5 SNPs are located in LF gene at nucleotide positions -190, -472, -292, -19 and +35 from the transcription startsite. Their MAF are: 0.13, 0.19, 0.19, 0.18 and 0.19 respectively. None of the polymorphic SNPs showedsignificant associations with milk yield of Egyptian buffalo what could be due to small sample size. Although nosignificant associations were detected between any of the investigated SNPs and milk yield in the current study,this study shades a light on some polymorphic sites in Egyptian buffalo that could be used as genetic markers.Hence, further association studies on these polymorphic sites in a larger sample size and against different traitsare recommended.

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