Lactococcus lactis subsp. cremoris C60 induces macrophages activation that enhances CD4+ T cell-based adaptive immunity

Bioscience of Microbiota, Food and Health

article

Suguru SAITO¹  Alato OKUNO⁴  Nanae KAKIZAKI⁴  Toshio MAEKAWA⁴  Noriko M. TSUJI⁴ 

[1] Division of Virology, Department of Infection and Immunity, Faculty of Medicine, Jichi Medical University;Department of Biomedical Sciences, Cedars-Sinai Medical Center;Faculty of Medicine and Dentistry, University of Alberta;Division of Cellular and Molecular Engineering, Department of Life Technology and Science, National Institute of Advanced Industrial Science and Technology;Department of Health and Nutrition, Faculty of Human Design, Shibata Gakuen University;iFoodMed Inc.;Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine;Division of Immune Homeostasis, Department of Pathology and Microbiology, Nihon University School of Medicine;Department of Food Science, Jumonji University

关键词: lactic acid bacteria;    macrophages;    CD4+ T cells;    IFN-γ;    IL-6;    IL-12/IL-23p40;    TNF-α;    probiotics;    antigen presentation;   

DOI  :  10.12938/bmfh.2021-057

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

Lactococcus lactis subsp. cremoris C60 is a probiotic strain that induces diverse functional modifications in immune cells. In this report, as a novel effect of C60 on myeloid lineage cells, we show that C60 enhances the immunological function of macrophages that consequently promotes CD4+ T cell activity in an antigen-dependent manner. Heat-killed (HK) C60 induced the production of pro-inflammatory cytokines in thioglycolate-elicited peritoneal macrophages (TPMs) much stronger than Toll-like receptor (TLR) ligand stimulation. The HK-C60 treatment also augmented the expression of antigen-presenting and co-stimulatory molecules, such as major histocompatibility complex (MHC) class II, CD80, and CD86, as well as antigen uptake in TPMs. These HK-C60-mediated functional upregulations in TPMs resulted in the promotion of CD4+ T cell activation in an antigen-dependent manner. Interestingly, the TPMs that originated from the mice fed the HK-C60 diet showed pre-activated characteristics, which was confirmed by the upregulation of cytokine production and antigen presentation-related molecule expression under lipopolysaccharide (LPS) stimulation. Furthermore, the antigen-dependent CD4+ T cell activation was also enhanced by the TPMs. This implied that antigen presentation activity was enhanced in the TPMs that originated from the HK-C60 diet mice. Thus, C60 effectively upregulates the immunological function of macrophages that directly connects to CD4+ T cell-based adaptive immunity.

【 授权许可】

CC BY