Differential Effects of Bacterial Lipopolysaccharide and Interferon-γ on Proliferation of Two Factor-Dependent Macrophage Cell Lines

Cell Structure and Function

Osamu Kohashi¹  Ariaki Nagayama²  Kazunori Ohki¹ 

[1] Department of Microbiology, Saga Medical School;Department of Microbiology, School of Medicine, Fukuoka University

关键词: macrophages;    cell line;    LPS;    IFN-γ;    proliferation;    signal transduction;   

DOI  :  10.1247/csf.17.161

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(30)Cited-By(2)We examined the responsiveness of two factor-dependent macrophage cell lines, BDM-1 and its subclone, BDM-1W3, to bacterial lipopolysaccharide (LPS), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) for their growth. LPS inhibited the M-CSF-dependent proliferation of BDM-1 cells but it had no effect on the proliferation of BDM-1W3 cells. LPS promoted DNA synthesis and supported the cell viability in the absence of CSFs in BDM-1 and BDM-1W3 cells, suggesting that the intracellular signals are transduced from the interaction of LPS with LPS-binding sites in BDM-1W3 cells as well as in BDM-1 cells. IFN-γ inhibited the proliferation of BDM-1 and BDM-1W3 cells. However, BDM-1 cells were more susceptible to the inhibitory effect of IFN-γ than BDM-1W3 cells. In contrast to LPS and IFN-γ, TNF-α did not inhibit the proliferation of BDM-1 and BDM-1W3 cells. These cell lines should be useful for studying the regulatory mechanisms in CSF-dependent macrophage proliferation.

【 授权许可】

Unknown