Antibodies Against Unusual Forms of Sialylated Glycans

Acta Naturae

article

Polina S. Obukhova¹  Marina M. Ziganshina²  Nadezhda V. Shilova¹  Alexander A. Chinarev³  Galina V. Pazynina³  Alexey Yu. Nokel¹  Anastasia V. Terenteva¹  Nailya R. Khasbiullina¹  Gennady T. Sukhikh⁴  Aligeydar A. Ragimov⁵  Emin L. Salimov⁴  Veronika I. Butvilovskaya⁶  Svetlana M. Polyakova³  Jaideep Saha⁷  Nicolai V. Bovin³ 

[1]Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health care of Russian Federation

[2]National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health care of Russian Federation

[3]Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

[4]National Medical Research Center for Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health care of Russian Federation, I.M. Sechenov First Moscow State Medical University of the Ministry of Health care of the Russian Federation, Sechenov University

[5]I.M. Sechenov First Moscow State Medical University of the Ministry of Health care of the Russian Federation, Sechenov University

[6]Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences

[7]Centre of Biomedical Research, Sanjay Gandhi PostGraduate Institute of Medical Science

关键词: sialylated glycans;    Kdn;    human natural antibodies;    pregnancy;    glycoarray;   

DOI  :  10.32607/actanaturae.11631

学科分类：生物技术

来源: Moskovskii Gosudarstvennyi Universitet im.M.V.Lomonosova/M.V.Lomonosov Moscow State University

【 摘 要 】

Previous studies have shown that in the blood of healthy donors (1) there are no natural antibodies against sialylated glycoproteins, which contain Neu5Acα (N-acetylneuraminic acid) as the most widespread form of human sialic acid, and (2) there is a moderate level of antibodies capable of binding unnatural oligosaccharides, where Neu5Ac is beta-linked to a typical mammalian glycan core. In the present study, we investigated antibodies against βNeu5Ac in more detail and verified the presence of Kdn (2-keto-3-deoxy-D-glycero-D-galacto-nonulosonic acid) as a possible cause behind their appearance in humans, taking into account the expected cross-reactivity to Kdn glycans, which are found in bacterial glycoconjugates in both the α- and β-forms. We observed the binding of peripheral blood immunoglobulins to sialyllactosamines (where “sialyl” is Kdn or neuraminic acid) in only a very limited number of donors, while the binding to monosaccharide Kdn occurred in all samples, regardless of the configuration of the glycosidic bond of the Kdn moiety. In some individuals, the binding level of some of the immunoglobulins was high. This means that bacterial Kdn glycoconjugates are very unlikely to induce antibodies to βNeu5Ac glycans in humans. To determine the reason for the presence of these antibodies, we focused on noninfectious pathologies, as well as on a normal state in which a significant change in the immune system occurs: namely, pregnancy. As a result, we found that 2/3 of pregnant women have IgM in the blood against Neu5Acβ2-3Galβ1-4GlcNAcβ. Moreover, IgG class antibodies against Neu5Acβ2-3Galβ1-4GlcNAcβ and Neu5Acβ2-6Galβ1-4GlcNAcβ were also detected in eluates from the placenta. Presumably, these antibodies block fetal antigens.

【 授权许可】

Unknown